Search Result of "Maneerat, T."

About 48 results
Img
Img

งานวิจัย

Screening of probiotic lactic acid bacteria and utilization for production of fermented shrimp (Kung-Som) (2009)

หัวหน้าโครงการ:ผศ.ดร.ศุภศิลป์ มณีรัตน์

ผู้ร่วมโครงการ:Imgดร.ศิริพร เรียบร้อย คิม, ผู้ช่วยศาสตราจารย์, Imgผศ.ดร.ศุภศิลป์ มณีรัตน์

แหล่งทุน:สำนักงานคณะกรรมการวิจัยแห่งชาติ

ผลลัพธ์:วารสาร (1) ประชุมวิชาการ (4)

Img
Img
Img

ผลงานตีพิมพ์ในวารสารวิชาการ

Probiotic lactic acid bacteria from Kung-Som: isolation, screening, inhibition of pathogenic bacteria (2010)

ผู้แต่ง:ImgSuppasil Maneerat, ImgDr.Siriporn Riebroy Kim, Assistant Professor,

วารสาร:

Img Img Img Img

Img

ผลงานตีพิมพ์ในวารสารวิชาการ

Survival and shelf life of Lactobacillus lactis 1464 in shrimp feed pellet after fluidized bed drying

ผู้แต่ง:ImgManeerat Wirunpan, ImgWanticha Savedboworn, ImgDr.Penkhae Wanchaitanawong, Associate Professor,

วารสาร:

Img Img Img

Img
Img
Img
Img
Img
Img
Img

ผลงานตีพิมพ์ในวารสารวิชาการ

Isoprenylated chromones from the stems of Harrisonia perforata

ผู้แต่ง:ImgCheenpracha, S., ImgChokchaisiri, R., ImgGanranoo, L., ImgManeerat, T., ImgRujanapun, N., ImgCharoensup, R., ImgLaphookhieo, S., ImgInjan, N., ImgDr.Somkiat Nokbin, Lecturer,

วารสาร:

Img

Img
Img
Img
Img

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Polyclonal Antibodies Specifi c to the Recombinant Coat Protein of Blackeye cowpea mosaic virus and Its Use in Disease Detection)

ผู้เขียน:ImgManeerat Koohapitagtam, ImgCharassri Nualsri

สื่อสิ่งพิมพ์:pdf

Abstract

The coat protein gene of Blackeye cowpea mosaic virus (BICMV-CP) was amplifi ed by reverse transcription polymerase chain reaction and cloned into the expression vector pQE-80L. This plasmid was transformed into Escherichia coli DH5? competent cells. The BICMV-CP gene was expressed as a fusion protein containing a fragment of 6xHis-tag. Bacterial cells were disrupted by repeated freezethawing three times and the BICMV-CP fusion proteins were purifi ed under denaturing conditions by affi nity chromatography with Ni-NTA Agarose. A sample of 500 ?g of purifi ed protein was mixed with Freund’s complete adjuvant at a ratio of 1:1 (volume to volume). Initially, the emulsion was subcutaneously injected into a New Zealand White rabbit, followed at weekly intervals by three additional immunizations with 500 ?g of the purifi ed protein mixed with Freund’s incomplete adjuvant. Bleeding was done every week during weeks 5-12 and titers of the antisera ranging from 800-51,200 were obtained. Up to a dilution of 1:320 of the BICMV-infected yardlong bean sap could be detected by indirect enzyme-linked immunosorbent assay. The produced antiserum reacted specifi cally with the BICMV-infected plant without any cross reaction with other virus species tested. However, a weakly positive reaction with Bean common mosaic virus could be observed.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 047, Issue 4, Jul 13 - Aug 13, Page 603 - 613 |  PDF |  Page 

Img

ที่มา:วิทยานิพนธ์ ปริญญาโท (จาก: บัณฑิตวิทยาลัย และ สำนักหอสมุด มก.)

หัวเรื่อง:การค้นคว้าด้วยตัวเอง หลักสูตร ปริญญาโทคหกรรมศาสตร์ ภาคพิเศษ

ผู้เขียน:Imgอนุสรณ์ ทองใหญ่

ประธานกรรมการ:Imgดร.ศิริพร เรียบร้อย คิม, ผู้ช่วยศาสตราจารย์

กรรมการร่วม:Imgดร.น้องนุช ศิริวงศ์, ผู้ช่วยศาสตราจารย์, ImgSuppasil Maneerat

Img

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Construction of Single-Chain Variable Fragment (scFv) Specific to Cucumber Mosaic Virus by Phage Display Technology)

ผู้เขียน:ImgManeerat Koohapitagtam, ImgSuang Rungpragayphan, Imgดร.รัชนี ฮงประยูร, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Cucumber mosaic virus (CMV) causes serious problems in economically important crops, especially members of the Solanaceae and Cucurbitaceae families. Serological detection of this virus by specific antibodies is required as a control measure as well as for quarantine investigation to ensure any components used for agricultural propagation, especially commercial seeds, are disease free. However, the selection of recombinant antibodies by phage display nowadays presents a real challenge to provide the antibodies that are urgently needed. In this research, an anti-CMV single-chain variable fragment (scFv) was constructed using a phage display system. Both heavy (VH) and kappa light chain variable (Vk) genes were amplified by RT-PCR from the hybridoma cell line CM2, secreting a monoclonal antibody (MAb) specific to both serogroup I and II of CMV. The VH and Vk amplified products, approximately 400 bp in length, were joined by a PCR overlapping extension method to generate the scFv gene. A recombinant phagemid pCANTAB5E harboring the scFv gene was constructed and transformed into Escherichia coli TG1. The bacterial transformants were rescued by helper phage M13 to produce phage-displayd scFv and the screening for CMV-specific scFv was carried out by ELISA. Three positive, recombinant clones (2C1, 6A1 and 1D4) which gave high signal-to-noise in ELISA were utilized in order to produce soluble antibodies. Western blotting and DNA sequencing were performed to characterize the scFv products. The result showed that all clones were identical and able to bind CMV of both subgroups. DNA comparisons showed that all the VH belonged to the J558.32 subgroup and JH2, while Vk belonged to Vk genes and JK2.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 043, Issue 2, Apr 09 - Jun 09, Page 330 - 338 |  PDF |  Page 

Img

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Ex-situ propagation of Pogostemon helferi (Hook. f.) Press using tissue culture and a hydroponics system)

ผู้เขียน:ImgManeerat Wangwibulkit, Imgดร.สรัญญา วัชโรทัย, รองศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

Pogostemon helferi (Hook. f.) Press, locally known as “dao-noi” is a rare Thai indigenous aquatic plant that is popular for use in aquaria and water gardens. To address its scarcity and to make the plant more readily available, two experiments were conducted to find the optimum conditions for ex-situ propagation. The first experiment aimed to determine the concentration of growth regulators for its micropropagation. Sterile explants were cultured using a combination of 3-indoleacetic acid (IAA) and 6- benzylaminopurine (BA) supplements in Murashige and Skoog media (MS). MS media containing IAA 0.1 mg/L and BA 3 mg/L induced the highest percentage of callus formation (93.33%). In addition, MS media with IAA added at 0.3 mg/L significantly (p < 0.05) increased the number of new shoots appearing and their length after 8 wk. The second experiment aimed to determine the optimum electrical conductivity of the nutrient solution and the humidity level for P. helferi growth within a hydroponics system. The results showed that an electrical conductivity of 1.6 mS/cm and 80% humidity were optimal for P. helferi growth and production to a marketable size. These methods should enable the production of P. helferi appropriate to support market demand and thus can reduce the current practice of harvesting wild plants in their natural habitat.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 050, Issue 1, Jan 16 - Feb 16, Page 20 - 25 |  PDF |  Page 

123